5x HOT FIREPol® Probe qPCR Mix Plus (ROX)

5x HOT FIREPol® Probe qPCR Mix Plus (ROX)

For in vitro use only

Description:

HOT FIREPol^®Probe qPCR Mix Plus (ROX) is optimized for real-time quantitative PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol^® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

HOT FIREPol^®DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Applications:

– Detection and quantification of DNA and cDNA targets

– Profiling gene expression

– Microbial detection

– Viral load determination

Mix Compatibility:

– Appilied Biosystems: QuantStudio^TM 12K Flex, ViiA^TM7, 7900HT, 7500, 7700, StepOne^TM & StepOnePlus^TM

– Stratagene: MX3000P^TM , MX3005P^TM

Mix Composition:

– HOT FIREPol^® DNA Polymerase

– 5x Probe qPCR buffer

– 15 mM MgCl₂ 1x PCR solution – 3 mM

– dNTPs

– ROX dye

ROX Dye:

ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.

Shipping and Storage Conditions:

Routine storage: -20°C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol^® Probe qPCR Mix Plus (ROX).

Applied BioSystems and ROX are trademarks of Applied Biosystems LLC, ViiA and QuantStudio are trademark of Life Technologies Corporation; Stratagene, MX3000P and MX3005P are trademarks of Stratagene Corporation.