SOLIScript 1-step Probe Kit

SOLIScript 1-step Probe Kit

Suitable for ROX-dependent and ROX-independent qPCR cyclers

For in vitro use only

Description:

SOLIScript 1-step Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube.

Applications:

– DNA/LNA hydrolysis probe based assays

– Suitable for singleplex or duplex reactions

Reagents Provided:

– 5x One-step Probe Mix

HOT FIREPol® DNA polymerase, reaction buffer,

dNTPs, 15 mM MgCl2 (1x qPCR solution – 3 mM MgCl2),

internal reference based on ROX dye

– 40x One-step SOLIScript Mix

SOLIScript Reverse Transcriptase, RiboGrip RNase

Inhibitor

– Water, nuclease free

Shipping and Storage Conditions:

Routine storage: -20 °C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.