Description
SolisFAST® Lyo-Ready qPCR Kit with UNG is an optimized solution for fast and sensitive amplification and quantitation of DNA targets with probe-based assays. The kit comes in 2 tubes: the glycerol-free 5x SolisFAST® Lyo-compatible qPCR Mix with UNG and 4x SolisFAST® Lyo Excipient Mix. The composition is optimized for lyophilization to cakes or beads.
5x SolisFAST® Lyo-compatible qPCR Mix with UNG is a 5x concentrated qPCR master mix suitable for real-time detection and quantitation of up to five DNA targets simultaneously. It contains dUTPs instead of dTTPs, as well as Salini UNG® Uracil-N-Glycosylase to prevent carry-over contamination from previous amplifications. The mix does not contain any lyophilization additives or excipients.
4x SolisFAST® Lyo Excipient Mix is a 4x concentrated blend of additives that protect reagents during lyophilization. In combination with the glycerol-free qPCR mix, it enables lyophilization to beads or cakes.
Applications
- Detection and quantification of DNA and cDNA targets
- Lyophilization to beads and cakes
- DNA/LNA hydrolysis probe-based assays
- Singleplexing and multiplexing (up to 5 plex)
- Microbial detection
- Viral load determination
- Genotyping
Properties
Sample type: DNA
Hot-start: yes, 30 sec to 3 min initial activation at 95 °C
Cycling speed: fast or ultra-fast
Product format: reagent kit
Detection type: probe-based
Reference dye: none
Compatible real-time instruments: The product is compatible with most qPCR cyclers. If using a ROX-dependent platform, add ROX in concentrations recommended by the platform manufacturer, or switch off ROX normalization prior to the run.
Kit Components
SolisFAST® Lyo-Ready qPCR Kit with UNG comprises:
- 5x SolisFAST® Lyo-compatible qPCR Mix with UNG:
SolisFAST® DNA polymerase, Salini UNG® Uracil-N-Glycosylase, nucleotides (dUTPs, dATPs, dCTPs and dGTPs), MgCl2 (5x mix - 16.5 mM, 1x mix – 3.3 mM), glycerol-free reaction buffer.
- 4x SolisFAST® Lyo Excipient Mix:
A proprietary mix of lyophilization additives that serve as cryoprotectants and stabilizers.
